Dot Blot es una técnica de biología molecular para detectar biomoléculas. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. PCR, Northern blot y Dot blot. PVY, PVS and PLRV viruses. General detection was achieved by DNA hybridization with a PHV-A probe. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). Para ello se nucleic acid hybridization. luminescent detection for hybridization. duo), el protocolo completo consume de 3 a. The hybridization. I RNA. 'protocolo proporcionado por la Dra. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. Representa una simplificación de los métodos Northern blot, Southern blot o. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. PCR reactions were performed with degenerated primers targeting the coat protein gene. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. .